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KMID : 0385520120250010076
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Analytical Science & Technology
2012 Volume.25 No. 1 p.76 ~ p.82
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Analysis of dutasteride in human serum by LC-MS/MS
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Nam Hye-Seon
Nam Kyong-Hee
Jung Su-Hee
Lee Jang-Woo
Kang Jin-Yeong
Hong Soon-Keun
Kim Tae-Sung
Jung Ki-Kyung
Kang Tae-Seok
Yoon Hae-Jung
Lee Kwang-Ho
Rhee Gyu-Seek
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Abstract
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The determination and confirmation of dutasteride in human serum was performed by a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Beclomethasone as an internal standard (I.S.) was added to the serum and the mixed sample was pretreated by liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE). The mass transitions of dutasteride and I.S. monitored in multiple reaction monitoring (MRM) were m/z 529.6→461.5 and m/z 409.3→391.2, respectively, and the retention times were 6.45 and 5.46 min, respectively. The calibration curve was linear in the concentration range of 0.5~30.0 ng/mL (R2= 0.9999) and the limit of quantitation (LOQ) was found to be 0.5 ng/mL. The recovery of dutasteride was shown to be 66~72%. The intra-day assay precision and accuracy were in the range 3.5~5.5% and 85.7~89.9%, respectively, and the interday assay precision and accuracy were in the range 4.2~5.8% and 90.8~95.8%, respectively.
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KEYWORD
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Dutasteride, LC-MS/MS, human serum,
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